Review



pgrna guideless  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Addgene inc pgrna guideless
    A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome <t>targeting</t> <t>sgRNA</t> <t>(dCas9/pgRNA-non</t> target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.
    Pgrna Guideless, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgrna guideless/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    pgrna guideless - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "A broad-host-range CRISPRi toolkit for silencing gene expression in Burkholderia"

    Article Title: A broad-host-range CRISPRi toolkit for silencing gene expression in Burkholderia

    Journal: bioRxiv

    doi: 10.1101/618413

    A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome targeting sgRNA (dCas9/pgRNA-non target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.
    Figure Legend Snippet: A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome targeting sgRNA (dCas9/pgRNA-non target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.

    Techniques Used: SDS-Gel, Purification, Plasmid Preparation, Expressing



    Similar Products

    pgrna guideless  (Addgene inc)
    90
    Addgene inc pgrna guideless
    A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome <t>targeting</t> <t>sgRNA</t> <t>(dCas9/pgRNA-non</t> target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.
    Pgrna Guideless, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgrna guideless/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    pgrna guideless - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome targeting sgRNA (dCas9/pgRNA-non target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.

    Journal: bioRxiv

    Article Title: A broad-host-range CRISPRi toolkit for silencing gene expression in Burkholderia

    doi: 10.1101/618413

    Figure Lengend Snippet: A) dCas9 was expressed from the chromosome of K56-2 under a rhamnose inducible promoter. Cells were grown to OD 600nm of 0.6 then induced with rhamnose for three hours. The soluble protein fraction was run on an 8% SDS gel. dCas9 was detected by an α-dCas9 antibody followed by a second antibody linked to alkaline phosphatase. The lane labelled Cas9+ was loaded with 5 ng of purified Cas9. B, C and D) Growth curves of B. cenocepacia K56-2∷dCas9 (B), dCas9 expressed with a non-genome targeting sgRNA (dCas9/pgRNA-non target) (C) and K56-2∷CTX1-rha, the vector control plasmid for the integration (D) in LB media show that expression of the chromosomally-encoded dCas9 induced with rhamnose up to 1% does not affect growth.

    Article Snippet: The following plasmids have been deposited to Addgene for distribution: pAH-CTX1-rha (#129390), pAH-CTX1-rhadCas9 (#129391), pAH-CTX1-rhadCas9-native (#129392), pAH25-SceI (#129389), pSCB2-sgRNA (#129463), pgRNA-guideless (#129464), and pgRNA-non-target (#129465).

    Techniques: SDS-Gel, Purification, Plasmid Preparation, Expressing